Alcohol effects on long-term cognition

Due to the importance of lifestyle especially diet on the functional integrity of the liver, I study the effect of a high-fat diet on the hepatocyte function and structure in mice from a new perspective. Consequently, the mice were divided into two groups the first group, n=8, fed with standard rodent chow, 10Kcal, and the other group, n=16, fed with a high-fat diet, standard chow plus 4% cholesterol and 50% corn oil. The mice were kept for eight weeks and later their livers, and serums were isolated and analyzed. Hematoxylin and eosin were used to dye the liver tissues and test the ballooning, inflammation, steatosis in the tissue. Also, the fluctuation of liver enzymes such as aspartate aminotransferase and alanine aminotransferase was checked. Also, Orange software was used to analyze the pictures of slides under a light microscope. The result revealed the in addition to the increase in the liver enzymes such as ALT and AST, inflammatory cells, ballooning, and macro-vesicles increased sharply in hepatocytes. Besides, using logistic regression noted that ALT enzyme increase in the serum is more related to HFD diet than AST. Using Cohens’d analysis for effect size, demonstrated there is a strong positive relationship between the high-fat diet and the factors I estimated above. Orange software could distinguish between the healthy liver tissues and the tissues with aggregations of fat inside the hepatocytes with 92% accuracy. In contrast to the meaningfulness of the date, the confidence intervals of the effect size were wide