Ruchi Singh

Dr,  National Institute of Pathology 

Location: India

Consulting Services

Journal Manuscript Scientific Writing Career Advice and Mentoring Conferences Research Consulting Grant Submission

Areas Of Interest

Leishmania (Leishmania) donovani Leishmaniases, Cutaneous Leishmaniasis Malaria Antimicrobial Drug Resistance Gene Expression Infection

Professional Narrative

Employment

Education

Referees

Publications

  1. Yadav P, Azam M, Ramesh V, Singh R*. (2023) Unusual Observations in Leishmaniasis—An Overview. Pathogens. 2023; 12(2):297. https://doi.org/10.3390/pathogens12020297
  2. Ghosh S, Kumar V, Verma A, Sharma T, Pradhan D, Selvapandiyan A, Salotra P, Singh R*. (2022). Genome-wide analysis reveals allelic variation and chromosome copy number variation in paromomycin-resistant Leishmania donovani. Parasitology research, 10.1007/s00436-022-07645-x.
  3. Yadav G, Singh R*. (2022). In silico analysis reveals the co-existence of CRISPR-Cas type I-F1 and type I-F2 systems and its association with restricted phage invasion in Acinetobacter baumannii. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2022.909886. 
  4. Dev PP, Kathuria S, Srivastava P, Singh R, Sharma S. (2022). Reverse Koebner phenomenon in annular cutaneous leishmaniasis. J Eur Acad Dermatol Venereol.  doi: 10.1111/jdv.18603.
  5. Upmanyu K, Haq QMR, Singh R*. (2022).Factors mediating Acinetobacter baumannii biofilm formation: opportunities for developing therapeutics. Current Research in Microbial Sciences. 100131, https://doi.org/10.1016/j.crmicr.2022.100131.
  6. Rao GR, Kishore Raja Ratna M, Singh R, Haritha K. (2022).
    Diagnosis of post kala azar dermal leishmaniasis in nonendemic areas: A Clinical Challenge. Clin Dermatol Rev. doi: 10.4103/CDR.CDR_110_20
  7. Dixit KK, Ramesh V, Upadhyay S, Singh AK, Singh OP, Sundar S, Singh R*, Salotra P. (2021). Utility of blood as the clinical specimen for the molecular diagnosis of Post kala-azar dermal leishmaniasis (PKDL). J Clin Microbiol. 59 (9): e00132-21: doi: 10.1128/JCM.00132-21.
  8. Verma S, Deep DK, Gautam P, Singh R*, Salotra P. Proteomic Analysis of Leishmania donovani Membrane Components Reveals the Role of Activated Protein C Kinase in Host-Parasite Interaction. Pathogens. 2021; 10(9):1194. https://doi.org/10.3390/pathogens10091194.
  9. Azam M, Upmanyu K, Gupta R, Sruthy KS, Matlani M, Savargaonkar D, Singh R*. Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis of Plasmodium falciparum and Plasmodium vivax and Its Comparison with Loopamp™ Malaria. Diagnostics. 2021; 11(9):1689. https://doi.org/10.3390/diagnostics11091689
  10. Dixit KK, Ramesh V, Gupta R, Negi NS, Singh R*, Salotra P. (2021). Real time fluorimetry loop mediated isothermal amplification (RealAmp) for diagnosis of leishmaniasis and as a tool for assessment of cure for post kala-azar dermal leishmaniasis (PKDL). Am J Trop Med and Hyg. 104(6):2097–2107. doi: 10.4269/ajtmh.20-1057.
  11. Joshi S, Dixit K, Sharma V, Ramesh V, Singh R*, Salotra P. (2021). Rapid multiplex loop-mediated isothermal amplification (m-LAMP) assay for differential diagnosis of leprosy and post kala-azar dermal leishmaniasis. Am J Trop Med and Hyg. 104(6):2085–2090.doi: 10.4269/ajtmh.19-0313.
  12. Joshi S, Sharma V, Ramesh V, Singh R, Salotra P. (2021) Establishment of a novel loop-mediated isothermal amplification (LAMP) assay for rapid detection of M. leprae in clinical samples. Ind J Dermatol Venereol and Leprol.  doi: 10.25259/IJDVL_248_19.
  13. Azam M, Gaind R, Yadav G, Sharma A, Upmanyu K, Jain M, Singh R*. (2021). Colistin resistance among multiple sequence types of Klebsiella pneumoniae is associated with diverse resistance mechanisms: A report from India. Front Microbiol. 12:609840. doi: 10.3389/fmicb.2021.609840.
  14. Ghosh S, Verma A, Kumar V, Pradhan D, Selvapandiyan A, Salotra P, Singh R*. (2020). Genomic and transcriptomic analysis for identification of genes and interlinked pathways mediating artemisinin resistance in Leishmania donovani. Genes (Basel). 17;11(11):1362. doi: 10.3390/genes11111362.
  15. Dixit KK, Singh R, Salotra P. (2020). Advancement in molecular diagnosis of post kala-azar dermal leishmaniasis. Ind J Dermatol. 65 (6): 465-472. DOI:10.4103/ijd.IJD_311_19  
  16. Upmanyu K, Matlani M, Yadav P, Rathi U, Mallick PK, Singh R*. (2020) Allelic variation of msp-3α gene in Plasmodium vivax isolates and its correlation with the severity of disease in vivax malaria. Infect Genet Evol.; 104530. doi:10.1016/j.meegid.2020.104530
  17. Ramesh V, Dixit KK, Sharma N, Singh R, Salotra P. (2020). Assessing the efficacy and safety of liposomal Amphotericin B and miltefosine in combination for treatment of Post kala-azar dermal leishmaniasis. J Infect Dis. 221(4) 608-617. doi: 10.1093/infdis/jiz486.
  18. Verma A, Nayek A, Kumar A, Singh R*, Salotra P. (2020). Elucidation of role of an acetyltransferase like protein in paromomycin resistance in Leishmania donovani using in silico and in vitro approaches. J Biomol Struct Dyn. 38(15):4449-4460.   doi: 10.1080/07391102.2019.1682674.
19.  Verma A, Ghosh S, Salotra P, Singh R*. (2019). Artemisinin-resistant Leishmania parasite modulates host cell defense mechanism and exhibits altered expression of unfolded protein response genes. Parasitology Research, 118(9):2705-2713. DOI : 10.1007/s00436-019-06404-9.

 
20.  Deep DK, Singh R, Kulshrestha A, Wajid S, Salotra P. (2018). Lipase precursor like protein promotes miltefosine tolerance in Leishmania donovani by enhancing parasite infectivity and eliciting anti-inflammatory responses in host macrophages. Antimicrob Agents Chemother.  doi:10.1128/AAC.00666-18.

 
21.  Dixit KK, Verma S, Singh OP, Singh D, Singh AP, Gupta R, Negi NS, Das P, Sundar S, Singh R, Salotra P. (2018). Validation of SYBR green I based closed tube loop mediated isothermal amplification (LAMP) assay and simplified direct-blood-lysis (DBL)-LAMP assay for diagnosis of visceral leishmaniasis (VL). PLoS Negl Trop Dis. 12(11):e0006922. doi: 10.1371/journal.pntd.0006922.

 
22.  Verma A, Bhandari V, Deep DK, Sundar S, Dujardin JC, Singh R, Salotra P. (2017).Transcriptome profiling identifies genes/pathways associated with experimental resistance to paromomycin in Leishmania donovani. Int J Parasitol. Drugs and Drug res. 7(3):370-377. doi: 10.1016/j.ijpddr.2017.10.004

 
23.  Deep DK, Singh R, Bhandari V, Verma A, Sharma V, Wajid S, Sundar S, Ramesh V, Dujardin JC, Salotra P.(2017) Increased miltefosine tolerance in clinical isolates of Leishmania donovani  is associated with reduced drug accumulation, increased infectivity and resistance to oxidative stress. PLoS Negl Trop Dis.  11(6):e0005641. doi: 10.1371/journal.pntd.0005641.

 
  1. Singh R*, Singh DP, Savargaonkar D, Singh OP, Bhatt RM,  Valecha N.(2017). Evaluation of SYBR green I based visual loop mediated isothermal amplification (LAMP) assay for genus and species specific diagnosis of malaria in P. vivax and P. falciparum endemic regions. J Vector Borne diseases. 54 (1):54-60. *Corresponding Author 

 

25.  Verma S, Singh R, Sharma V, Bumb RA, Negi NS, Ramesh V, Salotra P. (2017). Development of a rapid loop-mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection. BMC Infect Dis. 17(1):223. doi: 10.1186/s12879-017-2318-8.
  1. Mohapatra S, Ghosh A, Singh R, Singh DP, Sharma B, Samantaray JC, Deb M, Gaind R. (2016). Hemozoin Pigment: An important tool for low parasitemic malarial diagnosis. Korean J Parasitol. 54(4):393-7. doi:10.3347/kjp.2016.54.4.393.
  2. Gupta P, Sharma R, Chandra J, Kumar V, Singh R, Pande V, Singh V. (2016).  Clinical manifestations and molecular mechanisms in the changing paradigm of vivax malaria in India. Infect Genet Evol. 39:317-324. doi: 10.1016/j.meegid.2016.02.014.
  3. Ramesh V, Singh R, K Avishek, Verma A, Deep DK, Verma S, Salotra P. (2015). Decline in clinical effficacy of oral miltefosine in treatment of post kala-azar dermal leishmaniasis (PKDL) in India. PLoS Negl Trop Dis 9(10): e0004093. doi:10.1371/journal.pntd.0004093
  4. Ramesh V, Kaushal H,  Mishra AK, Singh R, Salotra P. (2015). Clinico-epidemiological analysis of post kala-azar dermal leishmaniasis (PKDL) cases in India over last two decades: a hospital based retrospective study. BMC Public Health  15:1092. DOI 10.1186/s12889-015-2424-8
  5. Singh R*, Singh DP, Gupta R, Savargaonkar D, Singh OP, Nanda N, Bhatt RM, Valecha N. (2014). Comparison of three PCR based assays for noninvasive diagnosis of malaria: detection of Plasmodium parasites in blood and saliva. Eur J Clin Microb Inf Dis. 33(9):1631-9. doi: 10.1007/s10096-014-2121-z.  *Corresponding Author 
  6. Gupta P, Singh R, Khan H, Raza A, Yadavendu V, Bhatt RM, Singh V. (2014). Genetic Profiling of the Plasmodium falciparum Population Using Antigenic Molecular Markers, The Scientific World Journal.  Article ID 140867 DOI:http://dx.doi.org/10.1155/2014/140867
  7. Kulshrestha A, Sharma V, Singh R, Salotra P. (2014). Comparative transcript expression analysis of miltefosine-sensitive and miltefosine-resistant Leishmania donovani. Parasitol Res.  113:1171–1184.
  8. Singh R*, Savargaonkar D, Bhatt R, Valecha N. (2013). Rapid detection of Plasmodium vivax in saliva and blood using loop mediated isothermal amplification (LAMP) assay. J Infect. 67(3):245-247. *Corresponding Author
34.  Mallick PK, Singh R*, Singh OP, Singh AK, Bhasin VK, Valecha N. (2013). Reduced heterozygosity at intragenic and flanking microsatellites of pfcrt gene establishes natural selection based molecular evolution of chloroquine-resistant Plasmodium falciparum in India. Infect Genet Evol. 20: 407-412. *Corresponding Author  
35.  Bhandari V, Kumar D, Verma S, Srividya G, Negi NS, Singh R, Salotra P. (2013). Increased parasite surface antigen-2 expression in clinical isolates of Leishmania donovani augments antimony resistance. Biochem Biophys Res Commun. 440:646-651.
  1. Mallick PK, Sutton PL, Singh R, Singh OP, Dash AP, Singh AK, Carlton JM, Bhasin VK.(2013). Microsatellite analysis of chloroquine resistance associated alleles and neutral loci reveal genetic structure of Indian Plasmodium falciparum. Infect Genet Evol.19:164-175.
  2. Olasehinde GI, Yah CS, Singh R, Ojuronbge OO, Ajayi AA, Valecha N, Abolaji AO, Adeyeba AO.(2012) Genetic diversity of Plasmodium falciparum field isolates from south western Nigeria. Afr Health Sci.12(3):355-361.
  3. Kumar D*, Singh R*, Bhandari V, Kulshrestha A, Negi NS, Salotra P (2012). Biomarkers of antimony resistance: need for expression analysis of multiple genes to distinguish resistance phenotype in clinical isolates of Leishmania donovani. Parasitol Res. 111(1):223-230. *Equal Contribution
  4. Srividya G, Kulshrestha A, Singh R, Salotra P. (2012) Diagnosis of visceral leishmaniasis: developments over the last decade. Parasitol Res. 110(3):1065-78.
  5. Kulshrestha A, Singh R, Kumar D, Negi NS, Salotra P. (2011) Antimony-resistant clinical isolates of Leishmania donovani are susceptible to paromomycin and sitamaquine. Antimicrob Agents Chemother. 55(6):2916-21.
  6. Singh R, Kumar D, Duncan RC, Nakhasi HL, Salotra P. (2010). Over-expression of Histone H2A modulates drug susceptibility in Leishmania parasites. Int J Antimicrob Agents 36(1):50-7.
  7. Anvikar AR, Singh DK, Singh R, Dash AP, Valecha N. (2010). Vivax malaria presenting with cerebral malaria and convulsions. Acta Parasitolo. 55(1):96-8.
  8. Valecha N, Pinto RGW, Turner GDH, Kumar A, Rodrigues S, Dubhashi NG, Rodrigues E, Banaulikar SS, Singh R, Dash AP, Baird JK. (2009). Case report: Histopathology of fatal respiratory distress caused by Plasmodium vivax malaria. Am J Trop Med Hyg 81(5):  758-62.
  9. Valecha N, Srivastava P, Mohanty SS, Mittra P, Sharma SK, Tyagi PK, Pradhan K, Dev V, Singh R, Dash AP, Sharma YD. (2009). Therapeutic efficacy of artemether-lumefantrine in uncomplicated falciparum malaria in India. Malar J. 8:107.
  10. Kumar D, Kulshrestha A, Singh R, Salotra P. In vitro susceptibility of field isolates of Leishmania donovani to Miltefosine and amphotericin B: correlation with sodium antimony gluconate susceptibility and implications for treatment in areas of endemicity. (2009). Antimicrob Agents Chemother. 53(2):835-8.
  11. Kumar D, Srividya G, Verma S, Singh R, Negi NS, Fragaki K, Kubar J, Salotra P. (2008) Presence of anti Lepp12 antibody : a marker for diagnostic and prognostic evaluation of visceral leishmaniasis. Trans Roy Soc trop Med and Hyg 102(2):167-71.
  12. Subba Raju BV, Singh R, Sreenivas G, Singh S, Salotra P. (2008) Genetic Fingerprinting and identification of differentially expressed genes in isolates of Leishmania donovani from Indian patients of Post Kala-azar Dermal Leishmaniasis. Parasitology. 135(1): 23-32.
  13. Ramesh V, Ramam M, Singh R, Salotra P. (2008). Hypopigmented Post-kala-azar Dermal Leishmaniasis. Int J dermatol. 47: 414-416. 
  14. Ramesh V, Singh R, Salotra P. (2007) Post kala azar dermal leishmaniasis- a critical appraisal. Trop Med Int Health. 12(7):848-851.
  15. Singh R, Ramesh V, Poonam Salotra. (2007) Recent advances in Post kala azar dermal leishmaniasis. Science and Culture.  73 (5-6):132-137.
  16. Singh R, Kumar D, Ramesh V. Negi NS, Singh S, Salotra P. (2006) High incidence of antimony refractoriness in Indian Kala azar is contributed by anthroponotic transmission via Post kala azar dermal leishmaniasis. J Infect Dis. 194: 302-306.
  17. Salotra P, Singh R. (2006). Challenges in the diagnosis of Post Kala Azar Dermal Leishmaniasis (PKDL). Ind J. Med Res.123: 295-310.
  18. Salotra P, Duncan R C, Singh R, Subba Raju BV, Sreenivas G, Nakhasi HL. (2006). Up regulation of surface proteins in Leishmania donovani isolated from patients of post kala-azar dermal leishmaniasis (PKDL). Microbes and Infection. 8:637-644.
  19. Salotra P, Singh R, Duncan R, Nakhasi H. (2005). Microarray based analysis of gene expression in drug resistant Leishmania donovani isolated from Kala azar. Clin. Microbiol. Infect.11:S2-47-48.
  20. Salotra P, Singh R. (2005). Rapid and reliable diagnostic test for visceral leishmaniasis. Ind J Med Res. 122: 464-467.
  21. Singh R, Subba Raju BV, Jain RK and Salotra P. (2005). Potential of Direct Agglutination Test (DAT) based on promastigote and amastigote antigens for sero diagnosis of Post Kala Azar Dermal Leishmaniasis.  Clin. Diag. Lab Immunol.  12:1191-1194.
  22. Sreenivas G, Subba Raju BV, Singh R, Selvapandiyan A, Duncan RC, Sarkar D, Nakhasi HL, Salotra P. (2004). DNA polymorphism in Leishmania donovani that distinguishes patient isolates of Kala-azar and Post kala-azar dermal leishmaniasis.J. Clin. Microbiol.9:844-848.
  23. Sreenivas G, Singh R, A. Selvapandiyan, Negi NS, Nakhasi HL, Salotra P. (2004). Arbitrary –primed PCR for genomic fingerprinting and identification of differentially regulated genes in Indian isolates of Leishmania donovani. Experimental Parasitology.116:110-118.
  24. Sreenivas G, Nasim AA, Singh R, Subba Raju BV, Bhatheja R, Negi NS, Salotra P. (2002). Evaluation of potential of amastigote derived antigen in diagnosis of visceral leishmaniasis. Br. J. Biomed. Sci. 59: 218-222.

Grants,Awards and Certifications

Grants :

As  Principal Investigator 

  1. Application of new tools for molecular diagnosis and surveillance of post kala azar dermal leishmaniasis and cutaneous leishmaniasis in India.
  2. Whole genome sequence analysis of drug sensitive and resistant Leishmania isolates originated from patients of kala-azar and post kala-azar dermal leishmaniasis. 
  3. Development of novel quantitative isothermal fluorescence based rapid detection method for diagnosis of malaria.
  4. Evaluation of immune status and parasite load in patients of post kala-azar dermal leishmaniasis (PKDL) in response to treatment with miltefosine and amphotericin B.
  5. Genomic analysis of virulence and antimicrobial resistance determinants of hypervirulent Klebsiella  pneumoniae, an emerging threat to public health.
  6. Identification and characterization of artemisinin resistance-associated gene(s) in Leishmania.
  7. Evaluation of diagnostic potential of Loop-mediated isothermal amplification for rapid diagnosis of malaria.
  8. "Basic research study for defining the antimony resistance in Leishmania isolated from patients” funded by UNESCO Loreal FWIS.  (2006-2008). 

Awards & Prizes :

S.No.

Name of Award

Awarding Agency

Year

1

Dr TD Chugh Award for best publication in biomedical sciences

National Academy of Medical Sciences

2021

2

Bill & Melinda Gates foundation travel award to attend World Microbe Forum, June 20-24, 2021

American Society of Microbiology (ASM)

2021

 

Major General Saheb Singh Sokhey Award-2015

Indian council of Medical Research

2017

3

BILL & MELINDA GATES foundation award for Young Investigator from India and SEA

International Society for Infectious Diseases

2016

4

Women Scientist award for best paper

National symposium on “Microbes in Health and Agriculture” organized by School of Life sciences, JNU, New delhi

2012

5

Shakuntala Amirchand award for best-published work in biomedical sciences -2006

Indian council of Medical Research

2009

6

UNESCO L’oreal fellowship for young women in life sciences

UNESCO L’oreal FWIS

2006

7

JRF and SRF (NET)

Council of Scientific and Industrial   Research, Govt. of India

1999 -  2004

8

Director’s medal for securing Highest marks in B.Sc

Dayalbagh Educational Institute, Agra

1995

9

Director’s Medal for securing the highest marks in B.Sc. Hons (Chemistry)

Dayalbagh Educational Institute, Agra

1996

Languages

Language Proficiency :

English (Excellent Spoken and Written)

Translation Services :

Hindi (Sanskritised Hindustani) to English (Excellent Spoken and Written) to Hindi (Sanskritised Hindustani) (Excellent Spoken and Written) English to English (Excellent Spoken and Written) to Hindi (Sanskritised Hindustani) (Excellent Spoken and Written)

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