Dr mohammad tahir Waheed

Dr.,  Department of Biochemistry, Quaid-i-Azam University, Islamabad, Pakistan 

Location: Pakistan

Consulting Services

Journal Manuscript Grant Submission Scientific Writing Research Consulting Biostatistics Data Science-Bioinformatics Admin and Customer Support Career Advice and Mentoring Case Consulting Pharma-CRO-Device Hospital-Pharmacy-Insurance Sales and Marketing

Areas Of Interest

Biopharmaceuticals Vaccines Kits, Diagnostic Test Antibodies Molecular Pharming Cancer Pharmaceuticals Antioxidants Nutrition Assessment Biochemistry Human Papilloma Virus

Professional Narrative

I have more than 15 years of experience as researcher in the field of transformation and plant-based vaccines/pharmaceuticals. I am skilled in mentoring and supervising MS and PhD thesis, manuscript and thesis writing, proof reading, editing, reviewing, and professional training.

Employment

Associate Professor, Quaid-i-Azam University, Islamabad, Pakistan 2012

Education

PhD, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria 2012

Referees

Dr. Andreas Lössl, BOKU, Vienna Austria.

“Dr.”

Dr. Hans-Peter Kaul, BOKU, Vienna, Austria.

“Prof.”

Publications

  1. Waheed MT, Malik MS, Saba K, Younus I, Ahmad N, Mirza B, Lössl AG (2022) Plant-based Vaccines against Livestock Diseases: Way to Achieve Several Sustainable Development Goals. In: Applications in Plant Biotechnology Focus on Plant Secondary Metabolism and Plant Molecular Pharming (ISBN: 9780367344467). p. 204-238. Science Publishers, an imprint of CRC Press/Taylor & Francis Group.
  2. Aslam N, Sameeullah M, Yildirim M, Baloglu MC, Yucesan B, Lössl AG, Waheed MT, Gurel E (2022) Isolation of 3β-HSD promoter from Digitalis ferruginea subsp. ferruginea and its functional characterization in Arabidopsis thaliana. Molecular Biology Reports 7:1-11.
  3. Zia A, Zahoor M, Abdullah, Nisar A, Batool N, Bibi A, Saba K, Ahmed A, Karataş A, Gürel E, Waheed MT (2022) De novo assembly and characterisation of chloroplast genomes of broccoli cvs. Marathon and Green sprout using next generation sequencing. Turkish Journal of Agriculture and Forestry 46(4):525-535
  4. Latif S, Gottschamel J, Syed T, Younus I, Gull K, Sameeullah M, Batool N, Lössl AG, Mariz F, Müller M, Mirza B, Waheed MT (2022) Inducible expression of human papillomavirus‐16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen. Biotechnology and Applied Biochemistry 69:596-611.
  5. Sameeullah M, Yildirim M, Aslam N, Baloğlu MC, Yucesan B, Lössl AG, Saba K, Waheed MT, Gurel E (2021) Plastidial Expression of 3β-Hydroxysteroid Dehydrogenase and Progesterone 5β-Reductase Genes Confer Enhanced Salt Tolerance in Tobacco. International Journal of Molecular Sciences 22(21): 11736.
  6. Saba K, Malik MS, Latif S, Ijaz F, Sameeullah M, Waheed MT (2021) Plant Molecular Pharming For Human Diseases. In: Frontiers in Molecular Pharming (Eds: Muhammad Sarwar Khan), ISBN: 978-981-5036-67-0, p. 226-266, Bentham Science Publishers.
  7. Ijaz F, Saba K, Latif S, Batool N, Malik MS, Shafqat K, Ali Z, Waseem S, Sameeullah M, Waheed MT (2021) Transformation of tobacco protoplasts using mesoporous silica nanoparticles. Wulfenia 28(10): 15-45.
  8. Abdullah, Henriquez CL, Mehmood F, Hayat A, Sammad A, Waseem S, Waheed MT, Matthews PJ, Croat TB, Poczai P, Ahmed I (2021) Chloroplast genome evolution in the Dracunculus clade (Aroideae, Araceae). Genomics 113(1):183-192.
  9. Ismail H, Ijaz MU, Bhatti MZ, Liaquat H, Nawaz N, Zafar I, Nassan MA, Waheed RM, Badr RH, Latif S, Malik MS, Dilshad E, Waheed MT, Batiha GE (2021) Effect of Phoenix dactylifera extracts on anti-inflammatory, anticoagulant, analgesic and antidepressant activities. Wulfenia 28(2): 1-16.
  10. Abdullah, Shahzadi I, Ali Z, Islam M, Naeem M, Mirza B, Lockhart P, Ahmed I, Waheed MT (2021) Correlations among oligonucleotide repeats, nucleotide substitutions and insertion-deletion mutations in chloroplast genomes of plant family Malvaceae. Journal of Systematics and Evolution 59(2): 388-402.
  11. Mehmood F, Abdullah, Ubaid Z, Shahzadi I, Ahmed I, Waheed MT, Poczai P, Mirza B (2020) Plastid genomics of Nicotiana (Solanaceae): insights into molecular evolution, positive selection and the origin of the maternal genome of Aztec tobacco (Nicotiana rustica). Peer J 8: e9552.
  12. Ahmad N, Raza G, Waheed MT,  Zahid Mukhtar (2020) Food Safety Issues and Challenges of GM Crops. In book: Policy Issues in Genetically Modified Crops: A Global Perspective. Edition: 1st, Chapter: 16, pp:  Publisher: Elsevier Academic Press.
  13. Abdullah, Henriquez CL, Mehmood F, Carlsen MM, Islam M, Waheed MT, Poczai P, Croat TB, Ibrar Ahmed (2020) Complete chloroplast genomes of Anthurium huixtlense and Pothos scandens (Pothoideae, Araceae): unique inverted repeat expansion and contraction affect rate of evolution. Journal of Molecular Evolution 88: 562–574.
  14. Abdullah, Henriquez CL, Mehmood D, Shahzadi I, Ali Z, Waheed MT, Croat TB, Poczai P, Ahmed I (2020) Comparison of Chloroplast Genomes among Species of Unisexual and Bisexual Clades of the Monocot Family Araceae. Plants 9(6): 737.
  15. Saba K, Sameeullah M, Asghar A, Gottschamel J, Latif S, Lössl AG, Mirza B, Mirza O, Waheed MT (2020) Expression of ESAT‐6 antigen from Mycobacterium tuberculosis in broccoli: An edible plant. Biotechnology and Applied Biochemistry 67(1): 148-157.
  16. Mehmood F, Abdullah, Shahzadi I, Ahmed I, Waheed MT, Mirza B (2020) Characterization of Withania somnifera chloroplast genome and its comparison with other selected species of Solanaceae. Genomics 112(2), 1522-1530.
  17. Abdullah, Mehmood F, Shahzadi I, Waseem S, Mirza B, Ahmed I, Waheed MT (2020) Chloroplast genome of Hibiscus rosa-sinensis (Malvaceae): Comparative analyses and identification of mutational hotspots. Genomics 112(1): 581-591.
  18. Abdullah, Waseem S, Mirza B, Ahmed I, Waheed MT (2020) Comparative analyses of chloroplast genomes of Theobroma cacao and Theobroma grandiflorum. Biologia 75: 761–771.
  19. Saba K, Gottschamel J, Younus I, Syed T, Gull K, Lössl AG, Mirza B, Waheed MT (2019) Chloroplast-based inducible expression of ESAT-6 antigen for development of a plant-based vaccine against tuberculosis. Journal of Biotechnology 305:1-10.
  20. Ismail H, Kayani SS, Kayani SI, Mirza B, Waheed MT (2019) Optimization of cell suspension culture of transformed and untransformed lettuce for the enhanced production of secondary metabolites and their pharmaceutical evaluation. 3 Biotech. 9(9): 339.
  21. Abdullah, Shahzadi I, Mehmood F, Ali Z, Malik MS, Waseem S, Mirza B, Ahmed I, Waheed MT (2019) Comparative analyses of chloroplast genomes among three Firmiana species: Identification of mutational hotspots and phylogenetic relationship with other species of Malvaceae. Plant Gene 19: 100199.
  22. Younus I, Ismail H, Rizvi CB, Dilshad E, Saba K, Mirza B, Waheed MT (2019) Antioxidant, analgesic and anti-inflammatory activities of in vitro and field-grown Iceberg lettuce extracts. Journal of Pharmacy & Pharmacognosy Research 7(5): 343-355.
  23. Malik MS, Afzal M, Farid A, Khan FU, Mirza B, Waheed MT (2019) Disease Status of Afghan Refugees and Migrants in Pakistan. Frontiers in Public Health 7: 185.
  24. Sameeullah M, Khan FA, Özer G, Aslam N, Gurel E, Waheed MT, Karadeniz T (2018) CRISPR/Cas9-mediated immunity in plants against pathogens. Current Issue in Molecular Biology 26: 55-64.
  25. Ismail H, Dilshad E, Waheed MT, Mirza B (2017) Transformation of lettuce with rol ABC genes: extracts show enhanced antioxidant, analgesic, anti-Inflammatory, antidepressant and anticoagulant activities in rats. Applied Biochemistry and Biotechnology 181(3): 1179-1198.
  26. Ismail H, Dilshad E, Waheed MT, Sajid M, Kayani WK, Mirza B (2016) Transformation of Lactuca sativa L. with rol C gene results in increased antioxidant potential and enhanced analgesic, anti-inflammatory and antidepressant activities in vivo. 3 Biotech 6(2): 215.
  27. Waheed MT, Sameeullah M, Khan FA, Syed T, Ilahi M, Gottschamel J, Lössl AG (2016) Need of cost-effective vaccines in developing countries: What plant biotechnology can offer? SpringerPlus 5: 65
  28. Dilshad E, Zafar S, Ismail H, Waheed MT, Cusido RM, Palazon J, Mirza B (2016) Effect of Rol Genes on Polyphenols Biosynthesis in Artemisia annua and Their Effect on Antioxidant and Cytotoxic Potential of the Plant. Applied Biochemistry and Biotechnology 179: 1456–1468.       
  29. Waheed MT, Ismail H, Gottschamel J, Mirza B and Lössl AG (2015) Plastids: the Green Frontiers for Vaccine Production. Frontiers in Plant Science 6: 1005.
  30. Fahad S, Khan FA, Pandupuspitasari NS, Ahmed MM, Liao YC, Waheed MT et al (2015) Recent developments in therapeutic protein expression technologies in plants. Biotechnology Letters 37(2): 265-279      .
  31. Hassan SW, Waheed MT, Müller M, Clarke JL, Shinwari ZK, Lössl AG (2014) Expression of HPV-16 L1 capsomeres with glutathione-S-transferase as a fusion protein in tobacco plastids: an approach for a capsomere-based HPV vaccine. Human Vaccines and Immunotherapeutics 10(10): 2975-2982.
  32. Arshad W, Waheed MT, Mysore KS, Mirza B (2014) Agrobacterium-Mediated Transformation of Tomato with rolB Gene Results in Enhancement of Fruit Quality and Foliar Resistance against Fungal Pathogens. PloS one 9(5): e96979.
  33. Hassan SW, Mehmood Z, Waheed MT, Lössl AG (2013) Towards generation of transplastomic tobacco, expressing a 35 kDa protein as an antigen: a step towards affordable plant made vaccine against Mycobacterium. Cloning and Transgenesis 3: 117.
  34. Gottschamel J, Waheed MT, Clarke JL, Lössl AG (2013) A novel chloroplast transformation vector compatible with the Gateway recombination cloning technology. Transgenic Research 22: 1273-1278.
  35. Clarke JL, Waheed MT, Lössl AG, Martinussen I, Daniell H (2013) How can plant genetic engineering contribute to cost-effective fish vaccine development for promoting sustainable aquaculture? Plant Molecular Biology 83: 33-40.
  36. Waheed MT, Gottschamel J, Hassan SW, Lössl AG (2012) Plant-derived vaccines: An approach for affordable vaccines against cervical cancer. Human Vaccines and Immunotherapeutics 8(3): 403-406.     
  37. Kozak K, Galek R, Waheed MT, Sawicka-Sienkiewicz E (2012) Anther culture of Lupinus angustifolius: callus formation and the development of multicellular and embryo-like structures. Plant Growth Regulation 66: 145-153.
  38. Hassan SW, Waheed MT, Lössl AG (2011) New areas of plant-made pharmaceuticals. Expert Review of Vaccines 10(2): 151-153.
  39. Lössl AG, Waheed MT (2011) Chloroplast-derived vaccines against human diseases: achievements, challenges and scopes. Plant Biotechnology Journal 09: 527-539.         
  40. Waheed MT, Thönes N, Müller M, Hassan SW, Gottschamel J, Lössl E, Kaul HP, Lössl AG (2011) Plastid expression of a double-pentameric vaccine candidate containing human papillomavirus-16 L1 antigen fused with LTB as adjuvant: transplastomic plants show pleiotropic phenotypes. Plant Biotechnology Journal 09: 651-660.
  41. Waheed MT, Thönes N, Müller M, Hassan SW, Razavi MN, Lössl E, Kaul HP, Lössl AG (2011) Transplastomic expression of a modified human papillomavirus L1 protein leading to the assembly of capsomeres in tobacco: a step towards cost-effective second-generation vaccines. Transgenic Research 20: 271-282.
  42. Andreas Lössl, Syed W. Hassan, Tahir M. Waheed, Mona Najafian Razavi and Elke Lössl (2011) Molecular Farming: The Production of Vaccines in Plants. In: Essays on Agriculture and Environment. Eva Maleviti and Costas Stathopoulos Eds., ISBN: 978-960-6672-99-6, p.35-44, ATINAR Publishers, Athens, Greece.

Grants,Awards and Certifications

Grants :

1. Isolation and regeneration of protoplasts of Nicotiana tabacum: optimization of tissue culture conditions for plastid-based vaccine production (2012). Funded by Higher Education Commission (HEC) of Pakistan.

2. Development of a cost-effective vaccine against cervical cancer - a prophylactic approach (2016). Funded by Eureka-Eurostars. Amount: Rs. 150,00,00 (as subcontract).

3. Metabolic engineering, metabolome and promoter analyses of 3β-HSD, P5βR1 and P5βR2 genes from digitalis plant species (2016), funded by Tubitak, Turkey (completed): acted as co-advisor on the project.

4. Development of a plant-based cost-effective edible vaccine against human papillomavirus infection and cervical cancer (2019). Funded by Higher Education Commission (HEC), Pakistan.

Awards & Prizes :

1. PhD with Distinction

Languages

Language Proficiency :

English (Excellent Spoken and Written)

Translation Services :

German to English (Basic Spoken and Written)

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